| meets Mad Madam Mim | 1965 | Bob Ogle, Paul Murry | Story | 32 | Western | 50 |
| Topolino e il Natale movimentato | 1965 | Giampaolo Barosso, Giovan Battista Carpi | Story | 19 | Italy | 37 |
| The Tragic Magic Touch | 1965 | Tony Strobl | Story | 5 | Western | 37 |
| The Hound of Basketville | 1965 | Vic Lockman, Tony Strobl | Story | 10 | Western | 34 |
| Pluto Blasts Off | 1965 | Tony Strobl | Story | 4 | Western | 32 |
| Dogs Will Be Dogs | 1965 | Jack Bradbury | Story | 5.5 | Western | 30 |
| Shoo-Guy | 1965 | Tony Strobl | Story | 4 | Western | 29 |
| The No-Snap Trap | 1965 | Vic Lockman, Tony Strobl | Story | 6 | Western | 25 |
| A Three Ring Mystery | 1965 | Tony Strobl | Story | 10 | Disney Studio (foreign market stories) | 23 |
| Topolino e la mania di Pluto | 1965 | Giampaolo Barosso, Massimo De Vita | Story | 16 | Italy | 22 |
| The Magic Wand | 1965 | Jack Bradbury | Story | 8 | Disney Studio (foreign market stories) | 19 |
| Pluto's Bone-Anza | 1965 | Carl Fallberg, Tony Strobl | Story | 8 | Disney Studio (foreign market stories) | 18 |
| 25th Anniversary Issue | 1965 | Tony Strobl | Cover | 1 | Western | 17 |
| Pluto's Busy Day | 1965 | Jack Bradbury | Story | 8 | Disney Studio (foreign market stories) | 16 |
| Prologo a "Arcitopolino" | 1965 | Gian Giacomo Dalmasso, Giuseppe Perego | Story | 31 | Italy | 14 |
| Untitled newspaper strip | 1965 | Roy Williams, Manuel Gonzales | Newspaper strip | 1 | Sunday pages (US) | 14 |
| Pluto stella della TV | 1965 | Giampaolo Barosso, Adriana Cristina | Story | 14 | Italy | 9 |
| Topolino e le mine a passeggio | 1965 | Ennio Missaglia, Giulio Chierchini | Story | 30 | Italy | 9 |
| Untitled newspaper strip | 1965 | Roy Williams, Manuel Gonzales | Newspaper strip | 1 | Sunday pages (US) | 9 |
| Untitled newspaper strip | 1965 | Roy Williams, Manuel Gonzales | Newspaper strip | 1 | Sunday pages (US) | 8 |
| Untitled newspaper strip | 1965 | Roy Williams, Manuel Gonzales | Newspaper strip | 1 | Sunday pages (US) | 8 |
| Untitled cover | 1965 | René Guillaume | Cover | 1 | France | 7 |
| Untitled newspaper strip | 1965 | Roy Williams, Floyd Gottfredson | Newspaper strip | 1 | Daily strips (US) | 7 |
| Untitled newspaper strip | 1965 | Roy Williams, Manuel Gonzales | Newspaper strip | 1 | Sunday pages (US) | 7 |
| Untitled cover | 1965 | René Guillaume | Cover | 1 | France | 6 |
| Seeing is Believing | 1965 | Jim Fletcher | Story | 6 | Disney Studio (foreign market stories) | 6 |
| Untitled newspaper strip | 1965 | Roy Williams, Floyd Gottfredson | Newspaper strip | 1 | Daily strips (US) | 6 |
| Une poursuite mouvementée | 1965 | Serge Segret | Game or puzzle | <1 | France | 5 |
| Untitled newspaper strip | 1965 | Roy Williams, Julius Svendsen | Newspaper strip | 1 | Daily strips (US) | 5 |
| Untitled newspaper strip | 1965 | Roy Williams, Manuel Gonzales | Newspaper strip | 1 | Sunday pages (US) | 5 |
| Untitled newspaper strip | 1965 | Roy Williams, Manuel Gonzales | Newspaper strip | 1 | Sunday pages (US) | 5 |
| Untitled cover | 1965 | René Guillaume | Cover | 1 | France | 4 |
| Untitled cover | 1965 | René Guillaume | Cover | 1 | France | 4 |
| Doucement, Pluto ! | 1965 | Serge Segret | Game or puzzle | <1 | France | 4 |
| Gare à toi, Pluto ! | 1965 | Serge Segret | Game or puzzle | <1 | France | 4 |
| Untitled cover | 1965 | René Guillaume | Cover | 1 | France | 4 |
| Devinette | 1965 | Serge Segret | Game or puzzle | <1 | France | 4 |
| Pluto e la corsa a "ostacoli" | 1965 | Pier Carpi, Pier Lorenzo De Vita | Story | 12 | Italy | 4 |
| Untitled newspaper strip | 1965 | Roy Williams, Floyd Gottfredson | Newspaper strip | 1 | Daily strips (US) | 4 |
| Untitled newspaper strip | 1965 | Roy Williams, Floyd Gottfredson | Newspaper strip | 1 | Daily strips (US) | 4 |
| Untitled newspaper strip | 1965 | Roy Williams, Floyd Gottfredson | Newspaper strip | 1 | Daily strips (US) | 4 |
| Untitled newspaper strip | 1965 | Roy Williams, Floyd Gottfredson | Newspaper strip | 1 | Daily strips (US) | 4 |
| Untitled newspaper strip | 1965 | Roy Williams, Floyd Gottfredson | Newspaper strip | 1 | Daily strips (US) | 4 |
| Untitled newspaper strip | 1965 | Roy Williams, Floyd Gottfredson | Newspaper strip | 1 | Daily strips (US) | 4 |
| Untitled newspaper strip | 1965 | Roy Williams, Floyd Gottfredson | Newspaper strip | 1 | Daily strips (US) | 4 |
| Untitled newspaper strip | 1965 | Roy Williams, Floyd Gottfredson | Newspaper strip | 1 | Daily strips (US) | 4 |
| Untitled newspaper strip | 1965 | Roy Williams, Floyd Gottfredson | Newspaper strip | 1 | Daily strips (US) | 4 |
| Luglio | 1965 | Massimo De Vita | Illustration | <1 | Italy | 3 |
| Untitled newspaper strip | 1965 | Roy Williams, Floyd Gottfredson | Newspaper strip | 1 | Daily strips (US) | 3 |
| Untitled newspaper strip | 1965 | Roy Williams, Floyd Gottfredson | Newspaper strip | 1 | Daily strips (US) | 3 |
| Untitled newspaper strip | 1965 | Roy Williams, Floyd Gottfredson | Newspaper strip | 1 | Daily strips (US) | 3 |
| Untitled newspaper strip | 1965 | Roy Williams, Floyd Gottfredson | Newspaper strip | 1 | Daily strips (US) | 3 |
| Untitled newspaper strip | 1965 | Roy Williams, Floyd Gottfredson | Newspaper strip | 1 | Daily strips (US) | 3 |
| Untitled newspaper strip | 1965 | Roy Williams, Floyd Gottfredson | Newspaper strip | 1 | Daily strips (US) | 3 |
| Untitled newspaper strip | 1965 | Roy Williams, Floyd Gottfredson | Newspaper strip | 1 | Daily strips (US) | 3 |
| Untitled cover | 1965 | | Cover | 1 | Holland | 2 |
| Untitled cover | 1965 | | Cover | 1 | Holland | 2 |
| Untitled cover | 1965 | Giuseppe Perego | Cover | 1 | Italy | 2 |
| Almanaque Tio Patinhas | 1965 | Jorge Kato | Illustration | 1 | Brazil | 1 |
| Untitled cover | 1965 | Jorge Kato | Cover | 1 | Brazil | 1 |